Additional Selective and Differential Cell Culture Media
NEOGEN Sorbitol MacConkey Agar, 5 kg
Sorbitol MacConkey Agar is used for the isolation of pathogenic Escherichia coli in a laboratory setting. Sorbitol MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans. Sorbitol MacConkey Agar is based on the formula by Rappaport and Henig. Originally developed for isolating enteropathogenic (EPEC) serotypes, O11 and O55, this medium is recommended for the isolation and differentiation of enterohemorrhagic E. coli O157:H7. Serotype O157 has been implicated in serious foodborne diseases. MacConkey Agar W/ Sorbitol contains sorbitol instead of lactose for differentiating enteropathogenic E. coli serotypes; these strains are typically sorbitol negative. MacConkey Agar W/ Sorbitol is recommended for food testing.For more information visit https://www.neogen.com/solutions/Microbiology/sorbitol-macconkey-agar
NEOGEN D/E Neutralizing Broth with Tween, 5 kg
D/E Neutralizing Broth is used to neutralize and determine the bactericidal activity of antiseptics and disinfectants. D/E Neutralizing Broth is not intended for use in the diagnosis of disease or other conditions in humans.D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer.For more information visit https://www.neogen.com/solutions/Microbiology/de-neutralizing-broth-with-tween
NEOGEN Pancreatic Digest of Gelatin, 500 g
Pancreatic Digest of Gelatin is an enzymatic digest of gelatin for use in preparing microbiological culture media in a laboratory setting. Pancreatic Digest of Gelatin is not intended for use in the diagnosis of disease or other conditions in humans.Pancreatic Digest of Gelatin provides a complex nitrogen source and is used as a media ingredient for fermentation studies. Pancreatic Digest of Gelatin alone is used to support the growth of non-fastidious microorganisms. Pancreatic Digest of Gelatin is deficient in carbohydrates and distinguished by low cystine and tryptophan content.For more information visit https://www.neogen.com/solutions/Microbiology/pancreatic-digest-gelatin
NEOGEN MacConkey Broth, 500 g
MacConkey Broth is used for the detection of coliform bacteria in milk and water. Conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for the selective enrichment of Escherichia coli from non-sterile samples. Conforms to Harmonized USP/EP/JP performance specification. Gelatin peptone provides a source of nitrogen, while lactose is a fermentable carbohydrate. Ox bile acts a selective agent inhibiting most Gram-positive organisms and bromocresol purple acts as a pH indicator. A color change from purple to yellow indicates growth of a bile tolerant, lactose fermenting organism such as Escherichia coli. According to the Harmonized USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar. For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-broth
NEOGEN Tryptone Glucose Extract (TGE) Agar, 500 g
Tryptone Glucose Extract (TGE) Agar is used for the cultivation and enumeration of microorganisms in dairy and bottled water products. Tryptone Glucose Extract (TGE) Agar is not intended for use in the diagnosis of disease or other conditions in humans. In the 1930’s, Bower and Hucker developed a medium for detecting bacteria in milk and other dairy products. Prickett used a glucose agar containing tryptone to study thermophilic bacteria in milk. This medium is known as Yeast Dextrose Agar. In 1948, the American Public Health Association (APHA) adopted Tryptone Glucose Extract Agar for use in testing milk and dairy products. Currently, APHA specifies Tryptone Glucose Extract Agar for the heterotrophic plate count procedure in testing bottled water. Tryptone Glucose Extract Agar is also known as Yeast Dextrose Agar.For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-glucose-extract-tge-agar
NEOGEN DNase Agar, 500 g
DNase Agar is used for the differentiation of microorganisms on the basis of deoxyribonuclease activity and is not intended for use in the diagnosis of disease or other conditions in humans.In 1956, Weckman and Catlin showed a correlation between increased DNase activity of Staphylococcus aureus and positive coagulase activity. Their research suggested DNase activity could be used to identify potentially pathogenic staphylococci. DiSalvo confirmed their results by obtaining excellent correlation between coagulase and DNase activity of staphylococci isolated from clinical specimens. Jeffries, Holtman, and Guse incorporated DNA in agar to study DNase production by bacteria and fungi. Polymerized DNA precipitates in the presence of 1N HCl, creating an opaque medium. Organisms that degrade DNA produce a clear zone around an inoculum streak.For more information visit https://www.neogen.com/solutions/Microbiology/dnase-agar
NEOGEN A-1 Medium, 500 g
A-1 Medium is used for the detection of coliform organisms in water and food in a laboratory setting. A-1 Medium is not intended for use in the diagnosis of disease or other conditions in humans.Since the early 1900’s enumeration of coliform organisms, specifically E. coli, have been used to determine water purity. Elevated temperature and most-probable-number (MPN) methods are routinely used for analysis of water and food samples for presence of fecal coliforms. A-1 Medium was formulated to hasten E. coli recovery and reduce the incidence of false positive cultures.In 1972 Andrews and Presnell developed A-1 Medium. This medium recovers E. coli from estuarine water in 24 hours instead of 72 hours, and in greater numbers without the pre-enrichment step. A-1 Medium can be used in a single step procedure for the detection of fecal coliforms in source water, seawater, treated wastewater, and foods.For more information visit https://www.neogen.com/solutions/Microbiology/a-1-medium
NEOGEN Tetrathionate Broth Base, 500 g
Tetrathionate Broth Base is used with iodine for the recovery of Salmonella spp. in a laboratory setting. Tetrathionate Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base is used as a selective enrichment for the cultivation of Salmonella spp. Salmonella organisms may be injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Mueller demonstrated the effectiveness of Tetrathionate Broth for enriching typhoid and paratyphoid bacilli while inhibiting coliform organisms. Using modified Mueller’s broth, Kauffmann increased the number of positive isolates. Tetrathionate Broth was used in studies for the poultry industry and in a collaborative study for rapid screening of Salmonella in food.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-broth-base
NEOGEN Blood Agar Base, 10 kg
Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. An inexpensive, general purpose agar base which, with the addition of 5-7% sterile blood, can be used to cultivate a wide range of microorganisms of indicative significance. Typical hemolysis patterns are obtained with this medium. Formula:Beef Extract - 10.0 g/LBalanced Peptone - 10.0 g/LSodium Chloride - 5.0 g/LAgar - 12.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/blood-agar-base
NEOGEN Mannitol Salt Agar, 500 g
Mannitol Salt Agar is used for the isolation of staphylococci in a laboratory setting. Mannitol Salt Agar is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to Harmonized USP/EP/JP Requirements.Chapman formulated Mannitol Salt Agar to isolate staphylococci by inhibiting growth of most other bacteria with a high salt concentration. Chapman added 7.5% Sodium Chloride to Phenol Red Mannitol Agar and noted pathogenic strains of staphylococci (coagulase-positive staphylococci) grew luxuriantly and produced yellow colonies with yellow zones. Non-pathogenic staphylococci produced small red colonies with no color change to the surrounding medium. Mannitol Salt Agar is recommended for isolating pathogenic staphylococci from samples, cosmetics, and microbial limit tests.For more information visit https://www.neogen.com/solutions/Microbiology/mannitol-salt-agar
NEOGEN BCYE Agar (Legionella Isolation Medium), 500 g
BCYE Agar is used (with appropriate supplementation) for the isolation of Legionella spp. from water and environmental samples. BCYE Agar is not intended for use in the diagnosis of disease or other conditions in humans.In 1977, McDade et al. identified Legionella pneumophila as the causative agent of Legionnaires’ disease, a multisystem disease manifested primarily by pneumonia. In 1978 a new medium, F-G Agar, resulted in improved growth of L. pneumophila, a very fastidious organism. Freely et al. modified F-C Agar by substituting yeast extract as a vitamin source and replacing starch with activated charcoal, producing Charcoal Yeast Extract (CYE) Agar. In 1980, Pasculle et al. reported that CYE Agar could be improved by the addition of ACES (N-2-acetamido-2-aminoethane sulfonic acid) buffer.For more information visit https://www.neogen.com/solutions/Microbiology/bcye-agar-legionella-isolation-medium
NEOGEN MacConkey Agar without Crystal Violet and Salt, 500 g
MacConkey Agar without Crystal Violet and Salt is used for the isolation and differentiation of Gram-negative enteric bacilli from specimens containing swarming strains of Proteus spp, and is not intended for use in the diagnosis of disease or other conditions in humans.MacConkey Agar is based on the bile salt-neutral red-lactose agar of MacConkey. The original MacConkey medium was used to differentiate strains of Salmonella typhosa from members of the coliform group. Formula modifications improved growth of Shigella and Salmonella strains. These modifications include the addition of 0.5% sodium chloride, decreased agar content, altered bile salts, and neutral red concentrations. The formula modifications improved differential reactions between enteric pathogens and coliforms.For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-agar-without-crystal-violet-and-salt
NEOGEN Casein Peptone Type I, 500 g
Casein Peptone Type I is an enzymatic hydrolysate of casein for use in preparing microbiological culture media in a laboratory setting and is not intended for use in the diagnosis of disease or other conditions in humans.Casein Peptone Type I is an enzymatic hydrolysate of casein, rich in peptones and amino acids (including tryptophane). This peptone can be utilized by most bacteria as a growth substrate.For more information visit https://www.neogen.com/solutions/Microbiology/casein-peptone-type-1
STEMCELL Technologies StemSpan™ Leukemic Cell Culture Kit, Size: 1 Kit
StemSpan™ Leukemic Cell Culture Kit has been developed for the in vitro culture and expansion of malignant cells and has been tested on chronic myeloid leukemia (CML) and acute myeloid leukemia (AML) samples. This optimized protocol allows users to expand, culture, and use malignant cells for drug screening. StemSpan™ Leukemic Cell Culture Kit includes the serum-free medium SFEM II (Catalog #09605), StemSpan™ CD34+ Expansion Supplement (10X; Catalog #02691), and small molecule UM729 (Catalog #72332).
NEOGEN EC Broth, 500 g
EC Broth is used for the detection of coliform bacteria at 37°C and Escherichia coli at 44.5°C. EC Broth is not intended for use in the diagnosis of disease or other conditions in humans.EC Broth (Escherichia coli Broth) is a selective enrichment broth designed for the isolation of coliforms, including E. coli, from water and food samples. It was the recommended medium of the American Public Health Association (APHA) and the AOAC.EC Broth is made selective for coliforms by the inclusion of Bile Salts in the dehydrated medium. The selective nature of this medium ensures that the growth of non-coliform bacteria is minimized. The medium is buffered by the addition of potassium phosphates and osmotically balanced by sodium chloride. The medium is used at 37°C for coliform organisms and 44.5°C is recommended for the isolation E. coli.For more information visit https://www.neogen.com/solutions/Microbiology/ec-broth